FIGURE 2.

Schematic representation of the interaction of actin with chromosome movement in the cell nucleus in budding yeast. Figures are not drawn to scale. (A) During the mitotic cell cycle, budding yeast chromosomes are arranged in a Rabl configuration. In interphase, the SPB is found opposite to the nucleolus. In the cytoplasm, actin filaments grow (dashed green arrow) and can contact the nuclear envelope. Chromosome movement is globally influenced by nuclear actin that acts in chromatin remodeler complexes and locally, at chromosome ends, by actin filaments that brush against the nuclear envelope. (B) Actin filaments transmit forces onto paired chromosomes during the meiotic pachytene state via LINC complexes resulting in vigorous chromosome movement. Although it was shown that nuclear actin has an influence on chromosome movement during meiosis (Lui et al., 2013), cytoskeletal actin filaments have a more drastic impact during this stage (Koszul et al., 2008).