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. 2017 May 19;10:144. doi: 10.3389/fnmol.2017.00144

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Copyright © 2017 He, Liu, Zhang, Liang, Dai, Zeng, Pei, Xu and Lan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of voluntarily running on amyloid β accumulation. (A) Immunofluorescent staining of Aβ1–16 in the control and running groups (200×, scale bar = 100 μm). (B) Histograms comparing the average Aβ1–16 fluorescence intensity in the cortex and hippocampus in the control and running groups (n = 6 per group). (C) Immunofluorescent staining of Aβ1–40 and Aβ1–42 in the control and running groups (250×, zoomed in three-fold, scale bar = 50 μm). (D) Histograms comparing the average Aβ1–40 and Aβ1–42 fluorescence intensity in the cortex and hippocampus in the control and running groups (n = 6 per group). (E) Comparison of the Aβ1–40 and Aβ1–42 levels detected with enzyme-linked immunosorbent assays (ELISAs). Datasets are expressed as means ± SD, n = 6. **P ≤ 0.01; ***P ≤ 0.001.