Table 1.

Candidate reference genes, primer sequences, and characteristics of PCR amplifications in Taihangia rupestris.

Gene	Gene ID	Description	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Size (bp)	E (%)
ACT	c26476	Putative actin family protein	AGCAAGCCTTTCGTCAGCAG	AACCAGCCTTCACCATTCCAG	179	98.2
ADF3	c29239	Actin-depolymerizing factor 3-like	GGAGACCAGCCAATGAAGAAT	CAAGACAAAGAGGACCTACCGA	213	99.9
EF1α	c16607	Elongation factor 1 alpha	CCCTGGGCAGATTGGAAACG	CCTCACAGCAAAGCGACCGA	235	102.3
GADPH	c30602	Glyceraldehyde-3-phosphate dehydrogenase	CCAATCAAGGTTGTCTCA	CATAGGTAGGAATGTCGG	184	96.8
HIS3	c38452	Histone H3	AAGCCCCACAGATACCGT	GTGTCCTCAAACAACCCAAC	212	94.1
ISP	c33198	Iron-sulfur cluster assembly protein	CGGAGCCAACAGGGAAAACT	TGGGTGAAGGGAAGGCAAAT	254	94.8
PP2A	c36176	Serine/threonine-protein phosphatase PP2A	ATTATGTGGACCGTGGCTAT	AATGCTGTCAGTGGGAAGTA	220	90.3
SPF	c34394	Splicing factor U2af large subunit B-like	GACCGTTGAAGAAGCCAGTA	GGTAGTCCACCCACAAAGATA	219	103.2
THS	c9010	Thiosulfate/3-mercaptopyruvate sulfurtransferase	CCGCCGTTTCTGCTTTAGG	AACACTCGGAACATCCACCAC	103	100.3
TMP50	c16729	Transmembrane protein 50	CACGGTTTCGTTCCTTCATTA	CTCCACTCGCCCTCTTCATA	117	92.1
TUA	c38618	Tubulin alpha-1	ATAAGTTGGTCGCTCAATGTCTA	TATCCTTCTCCGCAGGTTTC	159	104.5
UBC	c38569	Ubiquitin-conjugating enzyme E2	ATTGGGATGCCATAACTCTGAAG	ATTGGACCGCCTGATACGC	120	92.8
UFD1	c38829	Ubiquitin fusion degradation protein 1	ATTCAGGATAAGGAGGGGATT	ACGAAGACGCAGAACCAAGT	135	101.5
UBQ	c38440	Polyubiquitin	CGTGTTCAGCCAGCCATTCT	TCCCAAGTTCCAGGCATTCA	122	92.3
VAP32	c8974	Vacuolar protein sorting-associated protein 32	TCTTTTATTCTTTGCTCTGGTGA	TGAGACGCTTGAAATGCTTG	103	93.4
ACT2	−	Actin	GTACCCTCTTTCGGTGAGAATC	CCA ATCTACGAAGGTTATTCTC	100	92.8
TruPI	c30292	MADS-box protein PI	TCGTCATAGGATGGGGAACT	CGTCGTGGTCGTGAAGATTA	92	102.9
TruFBP24	c32480	MADS-box protein FBP24-like	GCTGATTAGGCTGGATAGAA	AAACAGAAGGAGGACGAGA	235	91.6