Table 2.
Expression stability analysis of reference genes assayed by NormFinder software.
| Ranking order | Staminate flower | Perfect flower | Floral tissue | Total | ||||
|---|---|---|---|---|---|---|---|---|
| Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | |
| 1 | UFD1 | 0.289 | HIS3 | 0.281 | HIS3 | 0.323 | UFD1 | 0.471 |
| 2 | ADF3 | 0.421 | ACT2 | 0.341 | UFD1 | 0.479 | HIS3 | 0.494 |
| 3 | HIS3 | 0.489 | ADF3 | 0.342 | TMP50 | 0.729 | ADF3 | 0.584 |
| 4 | ACT | 0.521 | TMP50 | 0.458 | ACT | 0.756 | TMP50 | 0.644 |
| 5 | SPF | 0.532 | SPF | 0.473 | ACT2 | 0.771 | SPF | 0.654 |
| 6 | UBQ | 0.576 | UFD1 | 0.506 | SPF | 0.783 | ACT | 0.716 |
| 7 | THS | 0.579 | THS | 0.568 | EF1α | 0.793 | EF1α | 0.720 |
| 8 | EF1α | 0.594 | EF1α | 0.575 | ADF3 | 0.810 | ACT2 | 0.780 |
| 9 | VAP32 | 0.658 | TUA | 0.618 | UBQ | 0.862 | THS | 0.788 |
| 10 | TMP50 | 0.664 | UBC | 0.632 | UBC | 0.866 | VAP32 | 0.862 |
| 11 | ACT2 | 0.722 | VAP32 | 0.680 | ISP | 0.945 | UBQ | 0.885 |
| 12 | ISP | 0.948 | UBQ | 0.716 | TUA | 0.954 | ISP | 0.953 |
| 13 | PP2A | 1.092 | ACT | 0.815 | VAP32 | 0.970 | PP2A | 1.062 |
| 14 | UBC | 1.169 | ISP | 0.916 | THS | 1.021 | TUA | 1.071 |
| 15 | GAPDH | 1.343 | PP2A | 0.993 | PP2A | 1.051 | UBC | 1.198 |
| 16 | TUA | 1.379 | GAPDH | 1.262 | GAPDH | 1.661 | GAPDH | 1.431 |