Table 4.
Expression stability ranking of the 16 candidate reference genes as calculated by RefFinder.
| Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Staminate flower (Better-Good-Average) | ||||||||||||||||
| Delta CT | UFD1 | ADF3 | HIS3 | ACT | SPF | EF1α | UBQ | THS | VAP32 | TMP50 | ACT2 | ISP | PP2A | UBC | GAPDH | TUA |
| BestKeeper | UFD1 | UBQ | VAP32 | THS | TMP50 | ADF3 | ACT | HIS3 | SPF | ACT2 | EF1α | ISP | PP2A | GAPDH | UBC | TUA |
| Normfinder | UFD1 | ADF3 | HIS3 | ACT | SPF | UBQ | THS | EF1α | VAP32 | TMP50 | ACT2 | ISP | PP2A | UBC | GAPDH | TUA |
| Genorm | HIS3|ADF3 | UFD1 | EF1α | ACT | SPF | UBQ | VAP32 | THS | TMP50 | ACT2 | ISP | PP2A | UBC | GAPDH | TUA | |
| Comprehensive ranking | UFD1 | ADF3 | HIS3 | ACT | UBQ | SPF | VAP32 | THS | EF1α | TMP50 | ACT2 | ISP | PP2A | UBC | GAPDH | TUA |
| Perfect flower (Better-Good-Average) | ||||||||||||||||
| Delta CT | HIS3 | ADF3 | ACT2 | TMP50 | SPF | UFD1 | THS | EF1α | TUA | UBC | VAP32 | UBQ | ACT | ISP | PP2A | GAPDH |
| BestKeeper | SPF | HIS3 | UFD1 | ACT2 | ADF3 | THS | TMP50 | UBQ | ISP | TUA | UBC | EF1α | VAP32 | GAPDH | PP2A | ACT |
| Normfinder | HIS3 | ACT2 | ADF3 | TMP50 | SPF | UFD1 | THS | EF1α | TUA | UBC | VAP32 | UBQ | ACT | ISP | PP2A | GAPDH |
| Genorm | HIS3|ADF3 | ACT2 | TMP50 | TUA | UFD1 | SPF | EF1α | THS | UBC | VAP32 | UBQ | ACT | PP2A | ISP | GAPDH | |
| Comprehensive ranking | HIS3 | ADF3 | ACT2 | SPF | TMP50 | UFD1 | THS | TUA | EF1α | UBC | UBQ | VAP32 | ISP | ACT | PP2A | GAPDH |
| Floral tissue (Better-Good-Average) | ||||||||||||||||
| Delta CT | HIS3 | UFD1 | TMP50 | SPF | ADF3 | ACT | ACT2 | UBQ | EF1α | UBC | ISP | VAP32 | TUA | THS | PP2A | GAPDH |
| BestKeeper | UFD1 | HIS3 | ADF3 | TMP50 | SPF | UBQ | ISP | TUA | ACT2 | VAP32 | UBC | ACT | EF1α | PP2A | THS | GAPDH |
| Normfinder | HIS3 | UFD1 | TMP50 | ACT | ACT2 | SPF | EF1α | ADF3 | UBQ | UBC | ISP | TUA | VAP32 | THS | PP2A | GAPDH |
| Genorm | SPF|UBQ | TMP50 | UFD1 | ADF3 | HIS3 | ISP | VAP32 | ACT2 | TUA | EF1α | ACT | UBC | THS | PP2A | GAPDH | |
| Comprehensive ranking | HIS3 | UFD1 | TMP50 | SPF | UBQ | ADF3 | ACT2 | ACT | ISP | EF1α | VAP32 | TUA | UBC | THS | PP2A | GAPDH |
| Total (Better-Good-Average) | ||||||||||||||||
| Delta CT | UFD1 | HIS3 | ADF3 | TMP50 | SPF | EF1α | ACT | ACT2 | THS | VAP32 | UBQ | ISP | PP2A | TUA | UBC | GAPDH |
| BestKeeper | UFD1 | ADF3 | UBQ | TMP50 | SPF | VAP32 | HIS3 | THS | ISP | EF1α | ACT | ACT2 | PP2A | UBC | TUA | GAPDH |
| Normfinder | UFD1 | HIS3 | ADF3 | TMP50 | SPF | ACT | EF1α | ACT2 | THS | VAP32 | UBQ | ISP | PP2A | TUA | UBC | GAPDH |
| Genorm | HIS3|UFD1 | ADF3 | TMP50 | SPF | ACT2 | EF1α | ACT | VAP32 | UBQ | THS | ISP | TUA | PP2A | UBC | GAPDH | |
| Comprehensive ranking | UFD1 | HIS3 | ADF3 | TMP50 | SPF | EF1α | UBQ | ACT | ACT2 | VAP32 | THS | ISP | PP2A | TUA | UBC | GAPDH |
The bold values mean the gene stability calculated by RefFinder, which integrates the DeltaCt, geNorm, Normfinder, and Bestkeeper algorithms.