Fig. 2.

Expression of the active form of GPIIb/IIIa on resting (a) or agonist-stimulated platelets: ADP 20 µM (b) and collagen 20 µg/ml (c) in whole blood samples obtained from SP MS patients and healthy controls. Analyses were performed in whole blood of healthy volunteers (control) and patients with SP MS. The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC-1/FITC. Statistical analysis was performed using Mann–Whitney U test