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. 2017 May 19;8:812. doi: 10.3389/fpls.2017.00812

FIGURE 5.

FIGURE 5

Hydrogen cyanamide (HC) altered the expression of ABA metabolism-related genes (A,B) and reduced the content of ABA in grapevine buds (C). HC down-regulated the expression of the ABA biosynthesis gene VvNCED2 (A) and up-regulated the expression of the ABA catabolism-related gene VvA8H3 (B) and reduced the content of ABA (C) in Thompson seedless grapevine buds. Gene expression analysis was performed by RT-qPCR, values were normalized against VvUBIQUITIN, and the content of ABA was determined as described in the Section “Materials and Methods”. Values are the average of three biological replicates with ± SD; (asterisk) Student’s t-test (α = 0.05).