FIGURE 1.

Effect of HIV-PIs on the growth behavior and morphology of F. pedrosoi conidial cells. (A) Different conidial cell densities (10³ and 10⁶ cells/ml) were incubated in the absence (control) or in the presence of indinavir, nelfinavir, ritonavir and saquinavir at 100 μM for 24 h. Subsequently, the mixtures were plated onto fresh solid medium without drugs. Image digitalization of the plates is shown. (B) In parallel, 10⁶ conidial cells/ml were treated (or not) with the HIV-PIs (100 μM) for 24 h and then analyzed by flow cytometry in order to measure cell size and granularity. Forward scatter (FSC) measurement is related to cell size and side scatter (SSC) measurement is related to the internal granularity and/or complexity of a cell. The values represent the mean ± standard deviation of three independent experiments performed in triplicate. Symbols (★72) indicate the experimental systems considered statistically significant from the control (P < 0.05, Student’s t-test).