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. 2017 May 19;8:918. doi: 10.3389/fmicb.2017.00918

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Copyright © 2017 Palmeira, Alviano, Braga-Silva, Goulart, Granato, Rozental, Alviano, Santos and Kneipp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HIV-PIs on the sterol synthesis of F. pedrosoi conidial cells. (A) Representative image of untreated (control) and HIV-PI-treated (100 μM for 24 h) conidial cells. For sterol analysis, the lipid extracts were loaded into HPTLC silica plates, separated using a mixture of solvents (hexane-ether-acetic, 80:40:2) and revealed by spraying a solution containing ferric chloride, water, acetic acid and sulfuric acid, which revealed the sterol as red-violet color spots. (B) Densitometric analyses of the lanosterol bands, expressed in arbitrary units. Symbols (★72) indicate the experimental systems considered statistically significant from the control (P < 0.05, Student’s t-test).