Fig. 5.

AraEXLB8 in situ hybridization. a Preserved RNA (red) after acridine orange treatment in root cells of a non hybridized RKN-inoculated A. stenosperma root (3 DAI). Drought-stressed roots of b A. gregoryi and c A. villosa with hybridization signals detected in cortical and epidermal root cells. d Well-watered (control) roots of A. gregoryi, with no hybridization signals. e RKN-inoculated roots showing evident signals in the vascular cylinder and surrounding endodermal cells, as well as some signals in epidermal cells, of A. stenosperma (3 DAI). f A. stenosperma inoculated roots (3 DAI) with no signals after T7 hybridization with the sense (T7) probe (technical control). ISH signals indicated by arrows. Bars = 20 µm