Figure 2.

LPS induces coordinate expression of PGE₂ pathway constituents needed to amplify IL-33 expression. A–E, we stimulated bmMFs with LPS (1.0 μg/ml) and collected RNA every 2 h until 8 h and monitored (A) IL-33, (B) EP₂ receptor, (C) EP₄ receptor, (D) COX-2, and (E) mPGES-1 mRNA levels. Data are presented as mean ± S.E. of three independent experiments. Statistical significance was determined using unpaired t test. p < 0.05 was considered statistically significant. *, p < 0.05.