Figure 3.

cAMP mediates potentiation of LPS-induced IL-33 production. A, IL-33 production by LPS-stimulated (1.0 μg/ml) bmMFs in the presence of 50.0 μm forskolin and 100 μm 8-bromo-cAMP (8-Br-cAMP). Data are presented as mean ± S.E. of three independent experiments. Statistical significance was determined using one-sample t test (comparing fixed 100%). p < 0.05 was considered statistically significant. *, p < 0.05. B, WB showing LPS-induced IL-33 protein levels in WT cells further enhanced by addition of PGE₂, 8-Br-cAMP, EP₂ and EP₄ agonists. C, Western blots showing the effects of PGE₂ and 8-Br-cAMP on IL-33 and COX-2 expression in cells from the indicated genotypes.