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. 2017 Mar 10;292(20):8236–8243. doi: 10.1074/jbc.M117.776419

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — Size exclusion chromatography and negative-staining electron micrograph. A, SEC profile on a Superdex 200 26/60 column of G. nivalis lectin affinity purified 4-2.J41gp41(BG505) shows a single well resolved peak corresponding to trimeric Env. A blue native-PAGE profile is shown in the right inset. In the left inset, a non-reducing PAGE with (+) and without (−) DTT is shown. The gp140 dissociates into gp120 and gp41 in the presence of DTT. B, NS-EM of purified trimeric Env. Reference-free two-dimensional class averages of 4-2.J41.HR1(BG505) (upper panel) shows presence of aberrant trimers (indicated with red arrows) along with well formed trimers. The 4-2.J41.gp41(BG505) (lower panel) shows well formed, compact trimers.