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. 2017 Mar 10;292(20):8236–8243. doi: 10.1074/jbc.M117.776419

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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Figure 3. — Conformational change of 4-2.J41.gp41(BG505) and its double cysteine mutant, DC4-2.J41.gp41(BG505) Envs with increased concentration of sCD4. To check the sCD4-induced conformational changes resulting from the exposure of epitopes for antibodies, 447-52D and 17b, the 4-2.J41.gp41(BG505) Env and the double cysteine mutant (I201C/A433C) Env, DC4-2.J41.gp41(BG505) incubated with increasing molar concentrations of sCD4 (1×, 5×, and 20×) followed by IP/Western blot analysis indicates a 20 m excess of sCD4 is needed to expose epitopes of 447-52D (top panel) and 17b (bottom panel).