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Thermostability of DC4-2.J41.gp41(BG505) trimer. A, DSC analysis of the purified DC4-2.J41.gp41(BG505) trimer reveals a melting profile with Tm value of 73 °C. B, IP/Western blot profile of DC4-2.J41.gp41(BG505) with bNAbs (PGT151 and PGT145) and non-NAbs (b6 and 447-52D) after incubation at 37 °C for different time points. C, intensity plot using ImageJ of bands obtained after IP/Western blot analysis in Fig. 5B, showing time-dependent changes in bNAbs and non-NAbs binding.
