Figure 3.

The amino terminus of MOAG-4 interacts with α-Syn. A, changes in the ¹H-¹⁵N HSQC spectra of MOAG-4 for increasing amounts of α-Syn. [α-Syn]/[MOAG-4] = 0, 0.2, 0.4, 0.7, 1, 2, and 4 (from red to blue). The inset in panel A is the binding curve for Gly²⁶. The solid line was obtained by global fitting of the chemical shift changes to Equation 1. B, backbone ¹³C′ (black) and ¹⁵N (magenta) chemical shift changes of MOAG-4 upon binding to α-Syn. The precision error is less than 0.03 ppm. C, structural propensity of MOAG-4 in the absence (red) and presence (blue) of α-Syn obtained from the backbone ¹H^N, ¹⁵N, and ¹³C′ chemical shifts.