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. 2017 Mar 10;292(20):8279–8290. doi: 10.1074/jbc.M116.774489

Figure 11.

Figure 11.

NHE3 PBMIImut and NHE3 Δ4 bind to casein kinase 2 (CK2) similarly to NHE3 WT. GST-tagged CK2 was used to pull down PS120/NHERF2 total cell lysates comparing PS120 cells (negative control), HA-tagged NHE3 WT, NHE3 PBMIImut, and NHE3 Δ4. Total expression and bound NHE3 WT, NHE3 PBMIImut, and NHE3 Δ4 were detected with monoclonal anti-HA antibody. The amounts of NHE3 PBMIImut and NHE3 Δ4 pulled down with GST-CK2 were similar to NHE3 WT. The arrow indicates the bound NHE3s. 5 μg of GST-CK2 was immobilized to 10 μl of glutathione-Sepharose 4B beads to pull down total lysates (1 mg/ml) of PS120 (negative) and NHE3 WT, NHE3 PBMIImut, and NHE3 Δ4 in PS120/NHERF2 cells. A representative experiment is shown, and these experiments were repeated three times with similar results.