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. 2017 Mar 10;292(20):8279–8290. doi: 10.1074/jbc.M116.774489

Figure 7.

Figure 7.

NHERF2-dependent NHE3 inhibition by elevated Ca2+ and cGMP/cGKII do not occur in the NHE3 PBMIImut and NHE3 Δ4 mutations. The NHERF2-dependent inhibitory effect of elevated Ca2+ (A) and cGMP (B) on NHE3 activity (Vmax) was studied. 4-Br-A23187 (0.5 μm; 5 min) inhibits NHE3 activity in NHE3 WT but not in NHE3 PBMIImut and NHE3 Δ4 in PS120/NHERF2 cells. 8-pCPT-cGMP (100 μm; 30 min) inhibits NHE3 activity (Vmax) in NHE3 WT but not in NHE3 PBMIImut and NHE3 Δ4 in PS120/NHERF2/cGKII cells. p values are comparisons of the 4-Br-A23187 or 8-pCPT-cGMP effect with untreated control (unpaired t tests). Experiments in A and B were repeated three times, and results are shown as mean ± S.D. Error bars represent S.D. NS, not significant.