Table 1.
Summary of NHE3 effects of PBM mutations from this study
All changes are relative to WT. NC, no change relative to WT; X, WT response prevented.
| Effect on NHE3 | NHE3 PBMIImut mutant (internal Class II PBM) | NHE3 Δ4 mutant (C-terminal Class I PBM) |
|---|---|---|
| Basal activity | Reduced | Reduced |
| Surface expression (%) | NC | Reduced |
| t½ PM | Decreased | NC |
| t½ total | NC | NC |
| Total amount | NC | Decreased |
| Stimulation by serum (not NHERF-dependent) | NC | NC |
| Inhibition by Ca2+ | X | X |
| Inhibition by cGMP | X | X |
| Basal Mf | Increased | Increased |
| Co-IP (NHERF2/NHE3) | X | X |
| SNX27, CaM-binding | NC | Decreaseda |
a Singh et al. (32) for SNX27.