Figure 6.
Pry1 preferentially binds saturated medium- and long-chain fatty acids and fatty alcohols. Ligand binding specificity was tested by a competition binding assay. Pry1 (100 pmol) was incubated with [3H]-palmitic acid (50 pmol) in the presence or absence of the indicated unlabeled fatty acid (50 pmol), arachidonic acid (C20:4), or the cysteinyl leukotriene LTC4, and binding of the radioligand was quantified. The chain length and degree of unsaturation of the tested fatty acids and the fatty alcohols (-OH) is indicated. Values represent means ± S.D. of three independent determinations. Asterisks denote statistical significance (*, p < 0.01; **, p < 0.001; ***, p < 0.0001). n.s., nonsignificant (p > 0.02); n.d., not determined.