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. 2017 Mar 27;292(20):8331–8341. doi: 10.1074/jbc.M116.772327

Figure 5.

Figure 5.

DDX41-regulated p21 expression requires a p53-dependent posttranscriptional event. A and B, Western blot analyses for the status of the indicated proteins in extracts of wild-type and p53-deficient HCT116 cells that were exposed for the indicated times to MG-132 (A) or BI-D1870 (B) after they were transfected with the control or the DDX41 siRNA. For densitometric and statistical analyses of p21 expression, refer to supplemental Fig. S7. C, Western blot analyses for the status of the indicated proteins in extracts of wild-type and p53-deficient HCT116 cells that were analyzed at the indicated times after γIR. D, Western blot analyses for the status of the indicated proteins in extracts of untreated (untr) and irradiated wild-type and p53-deficient HCT116 cells that were transfected before with either control or DDX41 siRNA. Samples were probed 8 h after γIR. All blots shown are representative results of at least three independent experiments. Low/high int., lower/higher scanning intensity.