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Assay performance of the cell-surface FRET assay in the HTRF mode (without washing steps) and TR-FRET mode (after washing) in A549 (upper tables) and HeLa cells (lower tables) was assessed by three parameters: coefficient of variation (%CV), signal-to-noise ratio (S/N) and the Z′ factor. Acceptable values were: %CV values < 10%, S/N > 8 and Z′ > 0.5. K: cell number in thousands.
