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Suppression of CD146/MCAM expression abolishes galectin-3-induced cytokine secretion. A–C, HUVECs were treated with control or CD146/MCAM siRNA for 36 h. The cells were then either lysed and analyzed for CD146/MCAM expression by CD146/MCAM immunoblotting (A) or further treated with galectin-3 (4 μg/ml) for 24 h before the levels of G-CSF (B) or IL-6 (C) in the culture medium were determined. *, p < 0.5 (unpaired t test).
