Figure 2.
The solution molecular mass(es) of β1a constructs. Full-length β1a (a), β1a-core (b), β1a-SH3/GK (c), and β1a-hook (d) constructs were analyzed by SEC-MALS. Proteins (0.1 mg) were applied to an analytical Superdex 200 size exclusion column. They were eluted in 20 mm Tris-HCl at pH 8.0 and 150 mm potassium chloride at room temperature. Samples were reduced with 1 mm dithiothreitol prior to application. The elution profile was monitored by the change in refractive index (continuous blue line). The molecular masses (kDa; secondary axis) corresponding to peaks are shown as discrete points. Bovine serum albumin (non-monomeric) was analyzed as a standard.