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. 2017 Apr 3;292(20):8436–8446. doi: 10.1074/jbc.M116.769380

Figure 2.

Figure 2.

Metformin bound to HMGB1 through its acidic tail. A, domain structure of HMGB1 and its mutants used in this study. B, purified His-tagged HMGB1 and its mutants were pulled down with the metformin beads and detected by immunoblotting with anti-His antibody. I, input (30%); C, control beads; M, metformin beads. C, full-length HMGB1 was pulled down with the metformin beads in the presence of the indicated concentrations of the acidic tail or HMGB1-ΔAT. Data shown are representative of three independent experiments with similar results.