Figure 8.

Metformin did not exhibit further inhibition when extracellular function of HMGB1 was blocked by anti-HMGB1-neutralizing antibody in the acetaminophen-induced liver injury model mice. Balb/c mice were intraperitoneally administered 400 mg/kg acetaminophen without or with 350 mg/kg metformin. Control antibody or anti-HMGB1 antibody at 5 mg/kg was simultaneously administered intravenously. A, serum ALT levels were measured 5 h after acetaminophen administration. Data are presented as the mean ± S.E. (n = 6–8). *, p < 0.05. NS, no significant difference. B, representative data of H&E staining in liver tissues 5 h after acetaminophen administration. Scale bar, 500 μm.