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. 2017 May 11;8:15236. doi: 10.1038/ncomms15236

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Copyright © 2017, The Author(s)

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Both light-induced phosphorylation and degradation of endogenous PIF3 are reduced in higher order ppk mutants. (a) Rapid mobility shift (phosphorylation) and (b) subsequent degradation in ppk1ppk2ppk3 (ppk123) and ppk1ppk2ppk4 (ppk124) triple mutants compared to wild-type (Col) in response to light. (c) Phosphorylation and degradation in artificial microRNA PPK1PPK2PPK3PPK4 (amiR-PPK1234) quadruple-knockdown mutant. Dark-grown (Dk) seedlings were irradiated with red light for 10 min (a, Rp), or the period indicated (b,c) before protein extraction and immunoblot analysis using anti-PIF3 antibodies. PIF3-P: phosphorylated PIF3; NS: nonspecific bands.