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. 2017 May 11;8:15236. doi: 10.1038/ncomms15236

Figure 7. Model depicting proposed mechanism of light-induced PIF3 phosphorylation.

Figure 7

Photoactivated phyB translocates rapidly into the nucleus and forms a trimolecular complex with PIF3 and one or more PPKs, which catalyse multisite transphosphorylation of PIF3. The LRB Cullin 3 E3 ligases then recognize phosphorylated PIF3, triggering concurrent ubiquitination and degradation of both PIF3 and phyB. X: potential additional factor contributing to Pfr-induced PIF3 phosphorylation in vivo, but missing from in vitro kinase assays performed here.