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. 2017 May 11;8:15234. doi: 10.1038/ncomms15234

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Copyright © 2017, The Author(s)

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Immunoblot assays showing the fluence-rate response of the blue light-dependent phosphorylation of CRY2 catalysed by PPK1 (a), PPK2 (b), PPK3 (c) and PPK4 (d) in HEK293T cells. Cells co-transfected to express CMV::Myc-CRY2 and indicated individual CMV::Flag-GFP-PPKs were exposed to the different fluence rates of blue light (0–100 μmol m⁻² s⁻¹) for 30 min. CRY2 and PPK were detected by immunoblots probed with anti-Myc or anti-Flag antibodies, respectively. Arrowheads and arrows indicate phosphorylated CRY2 (upshift bands) and unphosphorylated CRY2, respectively.