Figure 2. Proline catabolism via Prodh supports 3D growth.

(a) Representative pictures and size quantification of MCF10A H-Ras^V12 spheroids transduced with an inducible lentiviral CRISPR with or without guide for PRODH. CRISPR expression was induced with 0.1 μg ml⁻¹ doxycycline. Analysis was performed 5 days after PRODH knockdown (KD) induction. (b) Representative pictures and size quantification of MCF10A H-Ras^V12 spheroids with or without L-THFA treatment. Treatment was started at day 0. Analysis was performed at day 5 of treatment. Scale bar: 150 μm. The number of biological replicates for each experiment was n≥3. All error bars represent s.d. Two-tailed unpaired Student's T-test was performed. **P≤0.01; ***P≤0.001.