Figure 7. Prodh inhibition impairs lung metastases formation.

(a) Primary tumour weight, (b) the number of surface lung metastases and (c) representative pictures of ink-stained lungs with white tissue indicating metastases of BALB/c mice orthotopically injected with murine 4T1 breast cancer cells treated with vehicle or L-THFA at different concentrations for 16 days: 0 mg kg⁻¹ (n=36, 4 independent cohorts), 7.5 mg kg⁻¹ (n=9), 15 mg kg⁻¹ (n=8), and 30 mg kg⁻¹ (n=21, 3 independent cohorts). Treatment was started after primary breast tumours were formed. Scale bar: 0.5 cm. Two-tailed unpaired Student's T-test with Welch's correction was performed. **P≤0.01; ***P≤0.001. (d) Number of lung metastases per lung section and (e) representative pictures of H&E-stained lung sections of BALB/c mice orthotopically injected with murine 4T1 breast cancer cells treated with vehicle or L-THFA at 30 mg kg⁻¹ (n=5 per condition). Scale bar: 2.7 mm. Two-tailed Student's T-test with F-testing to confirm equal variance was performed. *P≤0.05. (f) Primary tumour weight, (g) the number of surface lung metastases and (h) representative pictures of ink-stained lungs (black) with metastases (white) of BALB/c mice orthotopically injected with murine EMT6.5 breast cancer cells treated with vehicle (n=9) or L-THFA at 15 mg kg⁻¹ (n=8) for 18 days. Scale bar: 0.5 cm. Two-tailed Student's T-test with F-testing to confirm equal variance was performed. *P≤0.05. All error bars represent s.e.m.