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. 2017 May 12;8:15319. doi: 10.1038/ncomms15319

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Copyright © 2017, The Author(s)

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Flow cytometric analysis of cells (a) Jurkat ATCC, (b) PBMC, (c) U937, (d) HT29, (e) Panc89, (f) L929Ts stained with PI (PI-positive fraction) treated with the 126 and 426 nm ZnO₂ nanoparticles (black and red columns, respectively) at various concentrations for 24 h, ‘0' concentration represents blank (control) samples containing the cell culture media but in absence of ZnO₂ nanoparticles. (g) Western blot analysis of PARP-1, representing apoptotic and necrotic events for U937, Jurkat ATCC, HT29, and L929Ts cell lines. Note: In this Figure, one representative experiment out of three with three parallel determinations each is shown. In addition, error bars, calculated using the STD function of Microsoft Excel, indicate the corresponding s.d.'s.