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. 2017 May 12;8:15262. doi: 10.1038/ncomms15262

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Copyright © 2017, The Author(s)

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(a) Western blot analysis of BRAF and CRAF protein expression and pERK activation in NRAS-mutated human melanoma cell lines (WM1361, WM852 and Sbcl2) transfected with the scrambled control (−) or short interfering RNA to BRAF and/or CRAF (siBRAF1 and siCRAF1). Total ERK and β-actin are used as a loading control. (b) Proliferation rate in WM1361, WM852 and Sbcl2 cells transfected with scrambled control (scr), BRAF siRNA (B1), CRAF siRNA (C1) or BRAFsiRNA/CRAFsiRNA (B1C1) was measured after BrdU incorporation during 3 hours. **P-value<0.01 and ***P-value<0.001 compared by Student's t-test. All data are represented as mean±s.d. (c) PLA showing NRAS/BRAF and NRAS/CRAF complexes in WM1361, WM852 and Sbcl2 cells. Numbers in white indicate the average number of dots per cell. Numbers between brackets represent s.d.'s. Scale bar, 100 μm.