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. 2017 May 12;8:15231. doi: 10.1038/ncomms15231

Figure 6. Phosphorylation stiffens the CTD.

Figure 6

(a) Kratky Plot (q2 × I(q) versus q) of averaged apo (blue) and phospho (red) MBP-CTD scattering curves. The more gradual rise in the high q region of the plot for the phospho-CTD than the apo-CTD indicates that the phospho-CTD is less flexible than the apo CTD. (b,c) Porod–Debye (q4 × I(q) versus q4) and Kratky Debye (q2 × I(q) versus q2) plots of the apo (blue) and phospho (red) scattering curves. The increased rise in the Porod-Debye plot for the apo-CTD compared to phospho-CTD indicates a phosphorylation dependent decrease in flexibility, as does the loss of the plateau in the Kratky–Debye plot for the phospho-CTD.