Figure 4. Small angle X-ray scattering reveals no significant change in pair-wise distances within CTD2′ upon extensive serine 5 phosphorylation.

(a) Raw scattering data for unphosphorylated CTD2′ (grey circles, bottom) and hyper-pSer5 CTD2′ (grey circles, top). Fits for unphosphorylated CTD2′ and hyper-pSer5 CTD2′ are shown superimposed on the raw data (solid black and red lines, respectively). (b) Representative pair-wise distance distributions for unphosphorylated CTD2′ (black) and hyper-pSer5 CTD2′ (red) calculated using the autoGNOM function in Primus qt, where the error bars represent the fit error.