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. 2017 May 12;8:15233. doi: 10.1038/ncomms15233

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Copyright © 2017, The Author(s)

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(a) Cβ and Cγ chemical shifts from the 3D CCCON spectrum of unphosphorylated CTD2′ demonstrate that, when resolved, individual proline side chain resonances show a nearly all-trans state. Blue and red bars represent the range of chemical shifts (mean±s.d.) for prolines in the trans and cis conformation, respectively. (b) Cβ and Cγ chemical shifts from the 3D CCCONH spectrum of hyper-pSer5 CTD2′ reveal dramatic trans to cis conformational switches in response to pSer5.