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. 2017 May 12;8:15233. doi: 10.1038/ncomms15233

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Copyright © 2017, The Author(s)

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(a) Representative kinetic traces of Ssu72 dephosphorylation of pSer5 in CTD2′ monitored by RT-NMR. (b) Apparent rate constants for pSer5 dephosphorylation reveal heptad-specific Ssu72 activities. The highest apparent Ssu72 activities are observed for pSer5 residues within heptads containing Asn7. Error bars represent the errors from non-linear least squares fitting. All fitting procedures are described in detail in Methods section.