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. 2017 May 12;8:15233. doi: 10.1038/ncomms15233

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(a) Ssu72 is shown as a ribbon diagram with α-helices in green and β-strands in gold. CTD peptides are shown as coloured sticks with carbon atoms shown in different colours: PDB code 4IMI (yellow), 4IMJ (blue), 3P9Y (salmon), and 3O2Q (magenta). The intra-molecular hydrogen bonds are shown in green dashed lines. The CTD residues are numbered based on consensus sequence and the following repeat residues are labelled with a prime. (b) The intra-molecular hydrogen bond network can be maintained even when Thr4 is replaced by Ser. (c) The replacement of Thr4 by Asn loses two intra-molecular hydrogen bonds. (d) An additional intra-molecular hydrogen bond can be formed (orange dashed line) when Ser7 is replaced by Asn.