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. 2017 May 12;8:15078. doi: 10.1038/ncomms15078

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Copyright © 2017, The Author(s)

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(a) Graph showing H1299 follower and leader cell growth over 3 days. ***P<0.001. (b) Colony formation assay of H1299 parental, follower and leader cells. (c) Cell cycle analysis of H1299 follower and leader cells that were serum starved, and then released for 20 h with normal growth media. (d) Colony formation assay of H1299 follower and leader cells. Cells were plated in conditioned media from FCM or LCM. Images taken 2 weeks after plating. (e) The colony size and number of colonies from d were measured using ImageJ software. *P<0.05, **P<0.01 using a Student's t-test. Error bars denote the s.e.m.