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. 2017 Mar 10;11(6):1318–1330. doi: 10.1038/ismej.2017.14

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Copyright © 2017 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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(a) Relative abundance (RA) of Legionella spp. in all samples under different water heater temperature settings (T in °C); (b) relative abundance (RA) of Legionella spp. in influent and control rig samples across time (t in month, mon). Relative abundance was calculated using Illumina 16S rRNA gene amplicon sequencing. Water use frequency and phase are indicated by color and shape, respectively. All influent samples were assigned a uniform temperature of 20 °C to facilitate comparison.