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. 2017 Feb 28;11(6):1386–1399. doi: 10.1038/ismej.2017.6

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Copyright © 2017 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Spatial variation in (a) methane flux from sediment mini-cores and (b) methane concentration in the bottom, water measured above the cores (<15 cm from sediment surface), plotted against seabed depth. The shaded area indicates where the conductivity–temperature–depth (CTD) profiles measured anoxic bottom water (~10 m from seabed). The Gaussian peak, 4-paramter regression line (black), 95% confidence (blue) and prediction (red) intervals are shown in a. Inset, oxygen concentration and methane concentration in the bottom water for the points shown in b. (c) Depth profiles of methanogenic potential (displayed on a logarithmic scale) measured in slurries at five discrete sediment layers from 0 to 25 cm in cores from 550 m (grey) and 650 m (black). As a result of this strong depth decay, all further experiments focused on the top 5 cm only. Hydrogen sulphide profile for a core from 550 m (650 m data unavailable) overlain in unfilled triangles.