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. 2017 Feb 28;11(6):1386–1399. doi: 10.1038/ismej.2017.6

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Copyright © 2017 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Methanogen community at five points across the seabed sediment. (a) Maximum likelihood tree of representative partial mcrA sequences (about 362 bp). Collapsed branches are indicated by a polygon. The mcrA gene of Methanopyrus kandleri (MKU57340) was used as the outgroup. Asterisks indicate local support values over 75%. The number in parenthesis following the ETNP_MG sequences indicates the % relative abundance of each OTU over the total number of OTUs (see Supplementary Table S2 for details). The bar represents 0.1 average nucleotide substitutions per base. (b) Principal coordinate analysis (PCoA) plot of the methanogen community based on the mcrA sequences and a maximum likelihood tree, and, constructed with the weighted Unifrac metric.