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. 2017 Feb 28;11(6):1386–1399. doi: 10.1038/ismej.2017.6

Table 2. Methane oxidation in long-term water incubations at 12 °C.

Latitude/longitude Sample water depth (m) Ambient oxygen (μmol l1) Ambient methane (nmol l1) 13C-DIC produced (nmol) Initial isotope spikes 15N-N2 produced (nmol)
13°21 N/91°23 W 195 1.9 3.1 0.2±0.03 264 nM 13CH4 NA
  200 1.5 2.8 0.6±0.34    
  205 ⩽1.4 4.4 0.1±0.09    
  210 ⩽1.4 3.4 0.20±0.22    
13°15 N/91°08 W 47 132.1 4.8 −0.1±0.06    
  226 ⩽1.4 2.1 −0.02±0.08    
13°16 N/ 91°08 W 65 11.4 2.4 0.2±0.02    
  228 ⩽1.4 3.9 0.3±0.11    
13°25 N/ 91°23 W 235 ⩽1.4 4.3 2.6±1.25a    
  235*     2.4±1.11a   80.5±30.11
13°16 N/ 91°08 W 322 ⩽1.4 14.8 10.4±0.18a 3.4 μM 13CH4 and for *samples also 11.4 μM 15NO2  
  322*     11.8±3.50a   16.0±2.64
  412 ⩽1.4 33.9 12.3±4.03a    
  412*     15.4±0.78a   14.5±4.11
13°16 N/91°08 W 264 ⩽1.4 9.4 1.3±1.20a    
  264*     12.0±6.00a   29.3±18.22
  256 ⩽1.4 6.1 11.2±0.00a    
  256*     0.9±1.37a   8.9±0.61

Abbreviations: NA, not applicable; OFN, oxygen-free nitrogen.

Mean values (± s.e., n=3). Samples marked with 'a' had a 2 ml OFN headspace throughout the incubation. Samples marked with * are those where 15N-NO2 (11.4 μmol l−1) was introduced and the production of 15N-N2 measured after 5 months. Where oxygen was at or below the limit of detection it was assumed to be ⩽1.4 μmol l−1.