Figure 1.

Effect of LAV-BPIFB4 on PKCα/eNOS signalling. Representative Western blots of (A) ex vivo C57BL/6 mouse mesenteric arteries, (B) HUVECs, and (C) vessels with (E+) or without (E−) endothelium, transfected with an empty vector (E) or vectors for the expression of WT BPIFB4 or LAV-BPIFB4. Graphs on the right show quantification of p-eNOS (S1177), p-PKCα (T497), p-BPIFB4 (S75), and BPIFB4. Values are means ± S.E.M., n = 6 experiments for A; n = 3 experiments for B and C. Statistics was performed using one-way ANOVA, following Bonferroni’s Multiple Comparison Test. *P < 0.05.