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. 2017 Apr 13;113(7):795–804. doi: 10.1093/cvr/cvx072

Figure 4.

Figure 4

LAV-BPIFB4 can activate endothelial function through an EDHF-mediated mechanism. (A) Western blot of ex vivo C57BL/6 mouse mesenteric arteries transfected with LAV-BPIFB4 or empty (E) expression vectors, in the presence or absence (−Ca2+) of external Ca2+. Right graphs show quantification of p-eNOS (S1177), p-PKCα (T497), p-BPIFB4 (S75), and BPIFB4. Values are means ± S.E.M., n = 6 experiments. Statistics was performed using one way ANOVA, following Bonferroni’s Multiple Comparison Test; *P < 0.05. Dose–response curves to ACh of mouse mesenteric arteries from ex vivo WT C57BL/6 mice (B,C) or from eNOS KO mice (D) transfected with empty vector (E) or with a vector for the expression of LAV-BPIFB4 in the absence of external Ca2+ ( − [Ca2+]ext) and in the absence or presence of the EDHF inhibitors APA + CTx (100 nM each). Values are means ± S.E.M., n = 11 experiments for B; n = 8 experiments for C and D. Statistics was performed using two-way ANOVA; *P < 0.05; **P < 0.01; #P < 0.05; ##P < 0.01 vs. after LAV + APA + CTx; §P < 0.05; §§P < 0.01 vs. before.