Figure 5.

The β₂AR-dependent cAMP response to local stimulation, dissipates from T-tubules early post-MI to crests at a later time point post-MI. (A) Representative curves of FRET response after local β₂AR stimulation either in T-tubule openings (dotted line) or on crest areas (black line). Local β₂ARs-dependent cAMP FRET response after agonist application (B) into T-tubules, sample numbers in control followed by chronological order of MI samples: N/n = 5/10, N/n = 6/15, N/n = 4/9, N/n = 5/17, or (C) onto crest, sample numbers in control followed by chronological order of MI samples: N/n = 5/11, N/n = 5/14, N/n = 4/10, N/n = 6/19. N, number of animals; n, number of cells. *P < 0.05, **P < 0.001, **P < 0.0001 as calculated with mixed ANOVA followed by Wald χ²-test.