Table 3.
Primers used to amplify and sequence DNA from C. flavida.
| Primer | Sequence (5′–3′) | Source | Ta (°C) |
|---|---|---|---|
| CHLOROPLAST MARKERS | |||
| SSC1-F | AAACAGAAGAGATCCGAGT | Designed by the authors | 51 |
| SSC1-1 | AATAGGCTATACTGACTGAA | ||
| SSC1-R | TGTTATTTTGTGTGCCGTTC | ||
| CP30-F | GTAGCAGCATGTATAAGAGCTGAA | Xi et al., 2012 | 57.5 |
| CP30-1 | AGCCCACATACGACGAAGTT | ||
| CP30-2 | TTTATTAGTAGGTCGATGAG | ||
| CP30-R | GCTACTCGGACTCGAACCGAGAT | ||
| SSC3-F | AAATCTCTTTCAACCGGAA | Designed by the authors | 49 |
| SSC3-1 | GATAGAACTATCCAGTTACA | ||
| SSC3-R | GCTCTATTTTGTTTATATTCGTC | ||
| NUCLEAR MARKERS | |||
| PAL-F | CACGTTACCACATTCAGCAACA | Designed by the authors | 56 |
| PAL-R | CCGCGAAACATCGATTAAGGG | ||