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. 2017 May 19;10:245. doi: 10.1186/s13071-017-2190-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Optical density readings for the control and treated group throughout this study. Heartworm antigen concentration was obtained using the DiroCHEK® Heartworm Antigen Test Kit. Sample wells were read using on an ELISA at 490 nm to quantify absorption and the concentration of heartworm antigen present in each sample. Heartworm antigen concentration was measured every 4 weeks throughout this study. The treated group exhibited significantly (P < 0.05) lower optical density readings compared to the control group on study days 168, 196, 224, 252, 279, 280 and 281