Figure 1.

 NONO interacts with PCDH19. (A) PCDH19 interaction with NONO was determined by co-immunoprecipitation assay using lysates from HEK293T cells expressing Myc-PCDH19, HA-NONO or both. Myc-PCDH19 was immunoprecitated and HA-NONO detected by western blotting with anti-HA antibody. (B) Lysates from HEK293T cells expressing Myc-tagged PCDH19 were immunoprecipitated and endogenous NONO detected by western blotting with anti-NONO antibody. (C) Endogenous NONO was immunoprecipitated from HeLa cells expressing Myc-PCDH19 and western blotted with anti-Myc antibody. Low level of Myc-PCDH19 immunoprecipitated with IgG was due to non-specific binding. Figure 1A–C was generated by cropping full-length western blots shown in Supplementary Material, Figure S10. Dotted lines separate the cropped areas of the western blots.