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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Immunol Lett. 2016 Mar 11;173:61–68. doi: 10.1016/j.imlet.2016.03.005

Fig. 4.

Fig. 4

Expression of LigA or LigB in L. biflexa controls deposition of MAC in the bacterial surface. Leptospires (2 × 108 bacteria) were incubated with 20% NHS for 60 min. After five washes, bacteria were incubated with polyclonal anti-human C3, anti-human C4 or anti-human MAC and then with the appropriate secondary antibodies for 30 min. Data are expressed as Mean Fluorescence Intensity (MFI) ± SE of 3 independent experiments each performed in triplicate on a Fluorescent Activated Cell Sorter (FACS) Canto II (Benton Dickson). Data were analyzed using ANOVA-Tukey’s Multiple Comparison Test *(p < 0.005).